Anti-tumor effect of cytosine deaminase genetherapy in situ combined with thermochemotherapy for colon cancer liver metastasis in nude mice
- VernacularTitle:原位基因治疗联合前药热化疗治疗结肠癌肝转移的实验研究
- Author:
Zhongdong ZOU
;
Baoming ZHANG
;
Chengjin LI
;
Lie WANG
- Publication Type:Journal Article
- Keywords:
Colorectal neoplasms?Neoplasms metastasis?Liver neoplasms?Cytisine deaminase?Flukytosine
- From:
Chinese Journal of Current Advances in General Surgery
2009;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the treatment effect of cytosine deaminase gene combined 5-FC thermochemotherapy for colon cancer liver metastasis in Nude Mice. Methods:The models of liver metastasis of colon cancer had been established by injecting human colon cancer cells Lovo into portal vein in 30 BALB/c nude mice,which were divided into transgenic group and non-transgenic group randomly. Transgenic group:virus supernatant was injected into abdominal cavity(0.2 mL/d,for 5 days); non-transgenic group:sodium chloride was injected into abdominal cavity(0.2 mL/d,for 5 days). In both groups 42 ℃ 5-FC was injected into the abdominal cavity (500 mg?kg-1?d-1,for 21 days). After treatment,all mice were sacrificed,and then PCR and RT-PCR were performed to detect the expression of targeted gene in carcinoma. The pathology changes of tumor were observed. Results:The targeted gene were detected in the tumor tissues. The rates of liver metastasis tumors were 26.7%(4/15),100.0%(15/15) in transgenic group and non-transgenic group respectively(P
