SYNTHESIS AND SECRETION OF HUMAN IFN-?B IN SACCHAROMYCE CEREVISIAE
- VernacularTitle:人?B干扰素在Saccharomyce cerevisiae中的合成和分泌
- Author:
Shengming WU
- Publication Type:Journal Article
- Keywords:
interferon;
promoter;
yeast strain;
synthesis;
secretion
- From:
Academic Journal of Second Military Medical University
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Modified HindⅢ fragment of IFN-?B gene was treated by nuclease S1 inserted into the HindⅢ site of plasmid YFD18 and fused with leader sequence of ?-factor gene,Resultant plasmid YFD33 was transformed into ?-type yeast strain Y33.The transforments could synthesize and secrete IFN-?B.More than 1.4?107 units/liter of interferon antiviral activity were in the medium and about 1.1?107 units/liter were in the cell.
