Evaluation of FHIT gene detection for early diagnosis lung cancer in sputum specimen
- VernacularTitle:痰液脱落细胞FHIT基因检测在肺癌早期诊断中的评价
- Author:
Penghui ZHUANG
;
Xiaogang JIANG
;
Chengen PAN
;
Dong SHANG
- Publication Type:Journal Article
- Keywords:
liquid-based cytology;
sputum specimen;
microsatellite instability;
loss of heterozygosity;
lung cancer diagnosis
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1981;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective By detecting microsatellite instability(MSI) and loss of heterozygosity(LOH) in FHIT gene in sputum specimen,we intended to evaluate the sensitivity of FHIT gene detection to early diagnosis of lung cancer and explore effective method to screen lung cancer.Methods Based on liquid-based cytology,we collected and isolated sputum specimens in high risk group,abstracted DNA,and detected MSI and LOH in FHIT gene.Results The positive rate of MSI or LOH at single locus was between 41.6% and 49.5%;the site D3S1300 was the highest and it was 49.5%.At least 1 site out of 3 sites that had abnormal microsatellite accounted for 72.3% in lung cancer patients,two sites that appeared to change accounted for 45.5%,which was significantly different compared to non-lung cancer patients(P
