Effect of continuously compressive pressure and human periodontal ligament cells on the differentiation of osteoclast-like cells in vitro
- VernacularTitle:压力与牙周膜细胞对体外诱导脐血单核细胞分化为破骨样细胞的影响
- Author:
Chengqiong WU
;
Hong ZHOU
;
Xiaorong WANG
- Publication Type:Journal Article
- Keywords:
mononucleared cell of umbilical cord blood;
human periodontal ligament cell;
continuously compressive pressure;
osteoclast-like cell
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1981;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of continuously compressive pressure(CCP) and human periodontal ligament cells(HPDLCs) on the differentiation of osteoclast-like cells(OLC) induced from umbilical cord blood cells in vitro,and to investigate the role of continuously compressive pressure and human periodontal ligament cells in alveolar bone rebuilding during orthordontic tooth movement.Methods Mononucleared cells of umbilical cord blood(HCMNCs) were separated by density gradient centrifugation,HPDLCs were isolated from human periodontal ligament by explanting enzymatic digestion with trypsin and collagenase.We also established transwell co-culture system with HCMNCs in the lower layer and HPDLCs in the upper layer.Group A: HCMNCs and HPDLCs were co-cultured with 150 kPa CCP for 1.5 hours on the model.Group B: only HCMNCs were cultured with the same CCP as Group A.Groups A'and B' were the respective control group of Groups A and B with no CCP exerted.The cell appearance was observed under the phase contrast microscope,and its identification was performed by histochemical analysis of tartrater-resistant acid phosphatase(TRAP).The capacity of bone resorption of the cell was assessed by lacunae forming ability on bone slice.Results HCMNCs in Group A began to fuse on the 2nd day,More positive multinucleated cells could be seen with TRAP staining and cortical bone pit formation on the 3rd day.Only a few multinucleated cells formed in the other groups,with no cortical bone pit formation.Conclusion HCMNCs can fuse into multinucleated OLC under CCP with the induction of HPDLCs.
