Effect of Particulate Matter 2.5 on the Proliferation and Secretion of NO,ET-1 in Vascular Smooth Muscle Cell of Rats in Vitro
- VernacularTitle:PM2.5对大鼠血管平滑肌细胞增殖及NO和内皮素分泌的影响
- Author:
Dang AO
;
Jianxin TAN
;
Kun CHENG
- Publication Type:Journal Article
- Keywords:
Air pollution;
Particulate matter;
Vascular smooth muscle cell;
Nitrogen monoxide;
Endothelin
- From:
Journal of Environment and Health
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of PM2.5 on the secretion of NO, ET-1 and the proliferation of rat vascular smooth muscle cell(VSMC) in vitro.Methods VSMCs were treated with PM2.5 collected from urban area of Zhanjiang,at the doses of 10,20,50,100 and 200 ?g/ml.After 24 hours of treatment, the levels of NO and ET-1 in the VSMCs were detected by nitrate reductase method and radioimmunoassay respectively, the proliferation of VSMCs was detected by MTT.Results At the concentrations of 10-200 ?g/ml, with PM2.5 increased, the level of NO in VSMCs decreased from(43.53 ?3.46)?mol/L to(29.28?2.28)?mol/L(F=18.89,P
