Preliminary analysis of side population phenotypes in human epithelial cells
- VernacularTitle:人表皮细胞中侧群细胞表型的初步分析
- Author:
Junmei CHENG
;
Ning REN
;
Qingdong ZENG
;
Juanhong SHI
- Publication Type:Journal Article
- Keywords:
Side population ?Epidermal stem cells ?ABCG2?integrin ?6? integrin?1
- From:Chinese Journal of Current Advances in General Surgery
2004;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore side population(SP) cells in human epithelial cells and to observe the expression of the universal stem cell marker ABCG2,epidermal stem cell markers ?6 integrin and ?1 integrin on SP cells.Methods:Epithelial cells were obtained by digesting human skins with Dispase II and Trypsin and stained with Hoechst33342 and PI.The SP cells were analyzed and sorted by the fluorescence-activated cell sorter.Then the expression of ABCG2,?6 integrin and ?1 integrin was analyzed by flow cytometry.Results:SP cells accounted for 0.2%-0.3% of total human epithelial cells.Only a small part of cells expressed ABCG2,integrin ?6 and integrin ?1 of both SP cells and total human epithelial cells detected by flow cytometry.The difference of positive rates between SP cells and total human epithelial cells was not significant.Conclusion:SP cells accounted for 0.2%-0.3% of total human epithelial cells.The difference of positive rates of stem cells marker ABCG2,integrin ?6 and integrin ?1 between SP cells and total human epithelial cells was not significant.
