Spinal fusion of lumbar intertransverse process in a goat by using tissue engineered bone with xenogeneic deproteinized bone as scaffold
- VernacularTitle:异种脱蛋白松质骨复合自体MSCs横突间成骨融合的作用
- Author:
Chunyang GAO
;
Ge SUN
;
Dongmei HAN
- Publication Type:Journal Article
- Keywords:
tissue engineered bone;
deproteinized bone;
spinal fusion
- From:
Orthopedic Journal of China
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To study the properties of xenogeneic deproteinized bone (DPB) used as scaffold in the bone tissue engineering and its application to the spinal fusion of the lumbar intertransverse process in a goat. [Methods]The deproteinized bone was derived from an adult pig femoral cancellous bone through physical and chemical treatments.The cell-material complex was observed under the inverted phase contrast microscope to evaluate the adhesion and the growth of the osteoblasts. The experimental model of the spinal fusion in the lumbar intertransverse process was produced in 24 male goats aged 6-8 months, which were divided into 3 groups according to different implant graft. All the samples were harvested at 4,8,12 weeks postoperatively, and a series of examinations were performed, including the radiography and the histomorphological assay.[Results]It showed that The DPB maintained natural pore network system, it hardly had any antigen, so it had good histocompatibility. In the spinal fusion model of lumbar intertransverse process,the cell-material complex could form cartilage and had a new bone formation in a multipoint way, the osteogenic process was almost the same as the auto-ilium osteogenesis and had a good mechanical strength.[Conclusion]The xenogeneic deproteinized bone is a good material in the bone tissue engineering, which can be used as an osteogenesis scaffold and provides a stable fusion.
