The effects of nicotine on the proliferation and fibronectin synthesis of human periodontal ligament cells cultured in vitro
- VernacularTitle:尼古丁对人牙周膜细胞增殖及纤维结合蛋白合成的影响
- Author:
Ying GAO
;
Chongtao LIN
;
Ying CHEN
- Publication Type:Journal Article
- Keywords:
PDLCs;
Nicotine;
Proliferation;
Fibronectins
- From:
Journal of Practical Stomatology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effects of nicotine on the proliferation and fibronectin(Fn) synthesis of human periodontal ligament cells(PDLCs) cultured in vitro,and to investigate the mechanism of the effects of smoking on the periodontitis.Methods:PDLCs were cultured in the presence of nicotine at various concentrations,the proliferation of cells was measured by MTT chromatometry,and the synthesis of Fn was measured by ELISA and reverse transcription-polymerase chain reaction(RT-PCR).Results:①MTT chromatometry:Nicotine inhibited the proliferation of PDLCs obviously(P
