Immune response of peptide-specific CD4~+ T cells in mice immunized by Dengue virus-specific peptide immunization
- VernacularTitle:登革病毒抗原肽免疫小鼠诱导特异性CD4~+ T细胞反应
- Author:
Zhiliang DUAN
;
Ruifeng MENG
;
Jinsheng WEN
- Publication Type:Journal Article
- Keywords:
Dengue virus;
Peptides;
ICS;
CD4+ T cells
- From:
Chinese Journal of Immunology
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the immunogenecity of four Dengue virus-specific peptides in mice.Methods:Each peptide of four Dengue virus-specific peptides (C45-57KLVMAFIAFLRFL,E396-408SSIGKMFEATARG,NS323-35YRILQRGLLGRSQ,and NS3141-155NREGKIVGLYGNGVV) was used to immunize BALB/c mice and C57BL/6 mice,respectively.Three weeks later,mice were killed and splelocytes were prepared.Splelocytes were stimulated by the same peptide and intracellular cytokine staining (ICS) assay was used to detect the percentages of peptide-specific IFN-? or IL-4 producing CD4+ T cells in total CD4+ T cells.Results:Peptide C45-57 induced peptide-specific IFN-?+ CD4+ T cells(0.72%?0.04% vs 0.04%?0.02%,P
