Effects of p,p’-DDE on Intracellular Calcium in Sertoli Cells of Rats in vitro
- VernacularTitle:p,p’-DDE对离体培养的大鼠睾丸支持细胞内钙离子的影响
- Author:
Chong WANG
;
Mingfen SONG
;
Hongwei ZHANG
- Publication Type:Journal Article
- Keywords:
Pesticide;
Sertoli cell;
Intracellular calcium;
Organochlorine pesticides;
p,p’-DDE
- From:
Journal of Environment and Health
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of p,p’-DDE on intracellular calcium in the Sertoli cells of rats in vitro. Methods The primary culture of the Sertoli cells separated from the testicle tissues of rats was conducted, and the cells were treated with p,p’-DDE at the doses of 10, 30 and 50 ?mol/L. Fura-2/AM was used as the probe to examine the changes of intracellular calcium by two-wavelength calcium fluorescence system. Results Compared with the control, in 50 ?mol/L group of p,p’-DDE, the intracellular calcium level significantly increased. Conclusion p,p’-DDE may disrupt intracellular calcium homeostasis which will induce calcium overload in Sertoli cells in the testicle tissues of SD rats.
