Expression of Calcitonin Gene Related Peptide in Spinal Cord and Dorsal Root Ganglion in Mercury Exposed Rats
- VernacularTitle:汞中毒大鼠脊髓及背根神经节降钙素基因相关肽的表达
- Author:
Jun ZHANG
;
Yongtian ZHOU
;
Xudong XU
- Publication Type:Journal Article
- Keywords:
Metal,heavy;
Mercurialism;
Neuropathic pain;
Calcitonin gene related peptide;
Spinal cord;
Dorsal root ganglion
- From:
Journal of Environment and Health
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of HgCl2 and drugs on the expression of calcitonin gene related peptide(CGRP) in the spinal cord and dorsal root ganglion(DRG).Methods Forty male SD rats(180-220 g) were randomly divided into 7 groups,5 in each treated group,10 in the control.Group A(high HgCl2),B(high HgCl2 +DMPS),C(high HgCl2+DMPS+ carbamzepine) were administrated with HgCl2 at the dose of 17 mg/kg(1/4 LD50),group D(low HgCl2),E(low HgCl2+DMPS),F(low HgCl2+NS) at the dose of 8.5 mg/kg(1/8 LD50),by gavage,once a day,the control group treated with normal saline(NS) in the same way.The sub-acute mercury poisoning models were developed within 18-22 days.Then five rats of control group and group A and D were killed and group B and E were treated with the chelator(DMPS) by intraperitoneal injection at the dose of 28 mg/kg for 2 periods.Group C was given DMPS+ carbamzepine and group F was given NS only.The carbamzepine was used at the dose of 16.82 mg/kg by gavage daily for 14 days.The spinal cord(L5-6) and the DRG of L5 and L6 were collected after the treatment finished.The immunohistochemistry SABC way was used to detect CGRP.Results The CGRP level in spinal cord and DRG of the rats exposed to HgCl2 increased significantly compared with the control group(P
