Experimental study of rabbit tendon early healing affected by mesenchymal stem cells composite grafts
- VernacularTitle:骨髓间充质干细胞及其复合物对肌腱早期愈合影响的实验研究
- Author:
Guiquan HAN
;
Shusen TANG
;
Xiaowei LIU
- Publication Type:Journal Article
- Keywords:
bone mesenchymal stem cells;
tendons;
healing
- From:
Orthopedic Journal of China
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To investigate the effect of rabbit BMSCs with fibrin sealant on accelerating early tendon healing.[Methods]The BMSCs were isolated and amplified.The rabbits were divided into experimental(E) and control(C) groups.The injury model was a sharp complete transection through the midsubstance of the achilles tendon.The experimental group was implanted with composite of BMSCs and fibrin sealant,The control group was operated with only fibrin sealant.Specimens were harvested at 1,3,6,and 12 weeks for analysis,which included evaluation of gross morphology,cell tracing,histological assessment,immunohistochemistry studies,morphometric analysis,and mechanical testing.[Results]The gross morphology of the tendons showed the experimental group had lesser adhesion and better reactiveness than that in the control group.The fibrin had degraded at 3 weeks.Cell tracing showed that the labeled bone marrow derived mesenchymal stem cells remained viable and presented in the intratendinous region for at least 6 weeks,becoming more diffuse at later timepoints.At 3 weeks,collagen fibers appeared more organized and there were better morphometric nuclear parameters in the treatment group.At 6 and 12 weeks,there were no differences between the groups with regard to morphometric nuclear parameters.Biomechanical testing showed improved modulus in the treatment group as compared with the control group at 3 weeks,but not at subsequent timepoint.[Conclusion]Intratendinous cell therapy with bone marrow derived mesenchymal stem cells following primary tendon repair can improve histological and biomechanical parameters in the early stage of tendon-healing.
