Isolation and purification of lily polysaccharide and its anti-tumor activity
- VernacularTitle:百合多糖的分离纯化及抗肿瘤作用
- Author:
Man MI
;
Fen LI
;
Lijun REN
;
Qibing MEI
- Publication Type:Journal Article
- Keywords:
lily polysaccharide;
isolation and purification;
anti-tumor activity
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1981;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To isolate and purify crude lily polysaccharide and observe anti-tumor activity of the isolated and purified polysaccharide.Methods Crude lily polysaccharide was extracted from lily by water extraction and ethanol precipitation.After dialyzing,de-proteining and freeze-drying,preliminary purification of crude lily polysaccharide was obtained.After being separated by anion-exchange chromatography,preliminary purification was further isolated and purified.Purified lily polysaccharide was given to the H22-bearing mice.The effects of purified lily polysaccharide on tumor weight and immune function were evaluated.Results Crude lily polysaccharide was isolated and purified successfully.After a Sephadex chromatography,the purified polysaccharide showed as a single peak,demonstrating its homogenicity.The purified lily polysaccharide showed an inhibitory effect on H22-bearing mice tumor growth.It significantly increased the weight of immune organs and improved the immune function of the mice.Conclusion The purified lily polysaccharide was homogeneous.It could inhibit H22 tumor growth and enhance non-specific immune functions in H22-bearing mice.This research has laid the foundation for further pharmacological study on lily polysaccharide.
