Age-related biological characteristics of rat adipose-derived stromal cells from different age stages
- VernacularTitle:不同年龄段大鼠脂肪间充质干细胞生物学特性的研究
- Author:
Tao TAN
;
Shujian QIN
;
Chao WANG
;
Wei SHAN
- Publication Type:Journal Article
- Keywords:
adipose derived stem cell;
age;
rat;
cell culture;
proliferation
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1981;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate biological characteristics of rat adipose-derived stromal cells(ADSCs) of different ages.Methods ADSCs were isolated by density gradient centrifugation from different age stages,and cultured in vitro.The adherent cells were preserved to passage,the purity of ADSCs was analyzed by immunocytochemistry method,and cell growth was observed,then proliferation capability and cell cycle were detected.Results All the ADSCs obtained from different age stages grew well and showed good morphology and growth characteristics.The proliferation rate of passage cells was higher than that of primary cells,but the proliferation activity reduced with aging,and cell cycle was prolonged.Conclusion The proliferation capability and activity of ADSCs decreased with aging.However,ADSCs of different age stages can all meet the needs of different patients for tissue engineering seed cells.
