Mechanism of vascular endothelial growth factor up-regulating intercellular adhesion molecule-1 via protein kinase C/nitric oxide pathway in the retina of diabetic rats
- VernacularTitle:糖尿病大鼠视网膜中VEGF经由PKC/NO上调ICAM-1表达的机制探讨
- Author:
Xiaoling ZHANG
;
Yanning DING
;
Yuan ZHU
;
Liang WEN
- Publication Type:Journal Article
- Keywords:
diabetes;
retina;
vascular endothelial growth factor(VEGF);
protein kinase C;
nitric oxide;
intercellular adhesion molecule-1(ICAM-1)
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1981;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate how vascular endothelial growth factor(VEGF) up-regulates intercellular adhesion molecule-1 via protein kinase C(PKC)/ nitric oxide(NO) pathway in the retina of diabetic rats.Methods All the rats were divided into 4 groups: normal,diabetes,diabetes+PKCI and control groups.Diabetes was induced by an intraperitoneal injection of STZ.PKC inhibitor GF109203X was injected intravitreally after 5 months of streptozotocin induced diabetes.NO was determined by nitrate reductase method.VEGF and intercellular adhesion molecule-1(ICAM-1) were measured by Western blot.Results VEGF and NO expressions increased obviously in the retina of diabetic rats compared with those in the normal group(P
