Construction and indentification of the recombinant vaccine of Attenuated Salmonella typhimurium containing pIRES2-EGFP-4-1BBL vector
- VernacularTitle:含pIRES2-EGFP-4-1BBL质粒减毒沙门氏菌株的构建与鉴定
- Author:
Jianxin YE
;
Yuanting ZHANG
;
Weichang CHEN
;
Xueguang ZHANG
;
Daming REN
- Publication Type:Journal Article
- Keywords:
4-1BBL gene;
Attenuation Salmonella typhimurium;
DNA vaccine
- From:
Chinese Journal of Immunology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
0.05).The transfected HepG2 cells by infection of SL3261 containing the vector were shown with GFP and the 930 bp target expression by RT-PCR.Conclusion:Attenuated Salmonella typhimurium SL3261 containing recombined eukaryotic expressing plasmid pIRES2-EGFP-4-1BBL is successfully constructed which can deliver recombinant plasmid into HepG2 cells.