Expression of ROR?t in pulmonary tissue of asthmatic mice and its relationship to airway inflammation
- VernacularTitle:哮喘小鼠肺组织中转录因子ROR?t的表达与气道炎症的关系
- Author:
Siming HU
;
Yaling LUO
;
Wenyan LAI
;
Peifen CHEN
- Publication Type:Journal Article
- Keywords:
Asthma;
ROR?t;
Airway inflammation
- From:
Chinese Journal of Immunology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the expression of ROR?t in pulmonary tissue of asthmatic mice and to investigate the association between the expression of ROR?t and the airway inflammation.Methods:Thirty female BLAB/c mice were randomly divided into the control group,asthmatic group and dexamethasone (Dex)-treated group.The asthma model was induced by classical method with ovalbumin(OVA).The concentration of IL-17 in bronchoalveolar lavage fluid (BALF) and serum was measured by enzyme-linked immunosorbent assay(ELISA).Airway inflammation was evaluated by HE staining.The expressions of IL-17,ROR?t mRNA and protein was measured by reverse transcription-polymerase chain reaction(RT-PCR) and Western blot respectively.Results:The level of IL-17,ROR?t mRNA and protein of asthmatic group was significantly higher than those of control group and Dexamethasone treated group (P
