Study on the expression of TLR4,NF-?B and TNF-? in the placenta of preterm birth and the intervention of NAC in infected BALB/c mice
- VernacularTitle:感染性早产小鼠胎盘TLR4,NF-?B和TNF-?的表达及NAC的干预作用研究
- Author:
Fuxi ZHAO
;
Zhiwei XING
;
Runhua LIU
- Publication Type:Journal Article
- Keywords:
Preterm birth;
Placenta;
TLR4;
NF-?B;
TNF-?;
NAC
- From:
Chinese Journal of Immunology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the expression of Toll-like receptor(4TLR4),nuclear factor -kappaB p65(NF-?Bp65) and tumor necrosis factor-alpha(TNF-?)in placenta of preterm birth of infected mouse model,as well as intervention of N-Acetylcysteine(NAC) in order to develop a novel strategy for prevention from premature birth.Methods:Expression of mRNA and protein of TLR4,NF-?Bp65 and TNF-? in the mouse placenta of premature birth was analyzed by using RT-PCR and immunohistochemistry.LPS injection was taken to develop the infectious mouse mode,and the incidence of premature birth was observed.The regimen of NAC intervention was performed and its modulation for placenta expression of TLR4,NF-?Bp65 and TNF-? was investigated.Results:It was shown that TLR4,NF-?Bp65 and TNF-? were expressed at a low level in control groups and in LPS model groups,their expressions were significantly increased when compared with the control groups(P
