The regulation of prednisone on expression of CⅡCA for accommodation of HLA in activated PBMCs
- VernacularTitle:强的松对CⅡTA调节HLA作用的影响
- Author:
Guiqin WANG
;
Lingxia LI
;
Tao LAN
- Publication Type:Journal Article
- Keywords:
HLA;
CⅡTA;
Prednisone;
Immune-suppression
- From:
Chinese Journal of Immunology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:CⅡTA is a transcriptional factor for transactivation of HLA expression.The aim of the study is to investigate the regulation of prednisone on inducible expression of CⅡTA in native PBMCs.Methods:Normal PBMC was stimulated with combination of IFN-? and PHA with or without the presense of prednisone.CⅡTA mRNA in the cells was amplified by RT-PCR,for analyzing transcriptive level in three designed groups of native PBMCs,activated PBMCs using the biomodulators and the triggered PBMCs in presense of prednisoloen.Results:Normal human PBMCs constitutively expressed CⅡTA,which was increased by stimulation of PHA and IFN-?.Prednisone significantly inhibited the inducible pattern of CⅡTA expression.Conclusion:Prednisone has showed inhibition effect on the expression of PBMC CⅡTA by descending transcription of the specialized transcriptional factor in activated PBMCs,whereby to downregulate the expression of HLA in PBMCs.
