Effects of Manganese on the Expression of Caspase-3 and Cytochrome-c in Spermatogenic Cells of Rats
- VernacularTitle:锰对大鼠生精细胞半胱氨酰天冬氨酸酶-3和细胞色素C表达的影响
- Author:
Xiulian CAI
;
Hai GUO
;
Guoxiu WANG
- Publication Type:Journal Article
- Keywords:
Manganese;
Rats;
Spermatogenic cell;
Caspase-3;
Cytochrome-c
- From:
Journal of Environment and Health
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of manganese on the expression of caspase-3 and cytochrome-c in the spermatogenic cells of rats. Methods Forty-eight male SD rats were randomly divided into the high dose,low dose exposure groups and the control groups,16 in each,were given manganese(MnCl2?4H2O) at 15,30 mg/kg and normal saline,I.P. once a day, 5 times a week,for 6 consecutive weeks. In the 4th and 6th weekend,the testis were collected,the expression of caspase-3 and cytochrome-c in the testis was determined by immunohistochemical methods (SABC). Results The caspase-3-positive-cell rate in the spermatogenic cells of rats in the high dose and low dose exposure groups increased significantly (P
