Effect of Volatile Chemicals in the Air of Newly Decorated Rooms on O~6-methylguanine-DNA Methyltransferase Expression in Lung,Liver,Kidney and Testicle Tissues of Mice
- VernacularTitle:居室空气中主要挥发性化学物对小鼠脏器O~6-DNA甲基转移酶表达的影响
- Author:
Junshan HE
;
Xinhua LU
;
Huifang CAO
- Publication Type:Journal Article
- Keywords:
Air pollution, indoor;
Volatile chemicals;
O6-methylguanine-DNA methyltransferase;
Immunohistochemistry
- From:
Journal of Environment and Health
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of volatile chemicals in the air of newly decorated rooms on O6-methylguanine-DNA methyltransferase (MGMT) expression in the lung, liver, kidney and testicle tissues of mice. Methods Forty Kunming mice were randomly divided into four groups and the imitate pollutants mixture of volatile chemicals in the air of newly decorated rooms was used as the exposed toxicants: low-dose exposure group (7.5 mg/m3), moderate-dose exposure group (15 mg/m3), high-dose exposure group (30 mg/m3), the control group (no exposure ). The mice were exposed 4 hours each day, for nine weeks. Streptavidin and biotechnology-peroxidase (SABC) immunohistochemical method was used to determine the MGMT expression in the lung, liver, testicle and kidney tissues. Results The expression of MGMT in the liver, lung, testicle tissues in the high, moderate and low-dose groups reduced significantly compared with the control group (P0.05). Conclusion The higher exposure of volatile chemicals from the air of newly decorated rooms may inhibit the MGMT expression in the liver, lung and testicle tissues of mice.
