The Expression of Estrogen Receptor Beta in the Lung Tissue of Mice Treaded with Benzo [a]pyrene Alone or Combined with Estrogen
- VernacularTitle:苯并[a]芘单独以及与雌二醇联合染毒对小鼠肺组织雌激素受体-?表达的影响
- Author:
Yunxiao ZHANG
;
Yufei ZHENG
;
Zhaoli CHEN
- Publication Type:Journal Article
- Keywords:
Respiratory system;
Benzo[a]pyrene;
Estrogen;
Estrogen receptor-beta
- From:
Journal of Environment and Health
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of estrogen receptor beta in the lung tissue of mice treaded with B[a]p alone or combined with estrogen (17 ?-estradiol, E2) in female Kunming strain mice and to explore the effect of estrogen in the lung cancer of mice induced by benzo[a]pyrene (B[a]P). Methods One hundred and twenty-five female Kunming mice were randomly divided into 5 groups: normal control group was group 1 ;group 2 was treated with subcutaneous injection of estrogen ( 900 ?g/kg); group 3 was given B[a]P(75 ?mol/kg) by gavage;the last two groups were given the same dosage of B[a]P (75 ?mol/kg)plus two different dosage of estrogen: 900 ?g/kg and 300 ?g/kg. After 8 weeks,there was a recovery period of 8 weeks. Then, the lung tissue was obtained by surgical resection. The expression of estrogen receptor-? gene and estrogen receptor-? protein of the lung tissue was detected by real-time PCR and Western blotting technology respectively. Results The expression of estrogen receptor-? protein and estrogen receptor-? gene in the B[a]P group and the B[a]P plus low dosage estrogen group was significantly higher than the normal group (P
