Animal model of heterotopic ossification induced by Achilles' tenotomy in Sprague-Dawley rat
- VernacularTitle:跟腱切断法诱导大鼠异位骨化动物模型的建立
- Author:
Junchang XU
;
Tao WU
;
Guihua WU
- Publication Type:Journal Article
- Keywords:
achilles tendon;
heterotopic ossification;
animal model;
rat
- From:
Orthopedic Journal of China
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To establish an animal model of heterotopie ossification(HO)induced by Achilles' tenotomy in Sprague-Dawley(SD)rat and to investigate its possible mechanism.[Method]Thirty six male SD rats were divided into 3 groups(n=12).In sham group,incision and suturation were performed only in the left leg.Partial tenotomy was performed in the left Achilles tendons in PAT group.In AT group,tenotomy was performed in the left Achilles tendons to establish animal model of HO.X-ray and histological examinations were made at 6 and 10 weeks after operation.[Result](1)No HO occurred in the sham and PAT groups.(2)In AT group,3 rats showed cartilage and bone formation on X-rays while the remaining 3 rats showed chondrification in histological examination at 6 weeks after operation.At 10 weeks all rats showed bone formation with trabecular bone.This kind of HO developed through a process of endochondral ossification.[Conclusion]Tenotomy is a simple,effective and feasible method to induce HO.
