Expression and puriffcation of fusion protein HSP2 in E.coli
- Author:
Ha Van Huan
- Publication Type:Journal Article
- Keywords:
protein HSP2, E.coli
- MeSH:
Escherichia coli;
proteins;
isolation & purification
- From:Journal of Vietnamese Medicine
2003;284(5):6-9
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
HSP2 recombinant in BL21 (DE3) pLysS strain was expressed at high level, but had varied in variable conditions from 22 - 370C. After simple steps of processing, HSP2 in soluble form could be purified by ion-exchange chromatography through CM-sepharose on FPLC system
