Effects of verapamil on the immediate-early gene expression of bone marrow mesenchymal stem cells stimulated by mechanical strain
- VernacularTitle:维拉帕米在应力对体外骨髓间充质干细胞早期反应基因蛋白表达中作用的研究
- Author:
Runguang LI
;
Jingfan SHAO
;
Mingfa WEI
- Publication Type:Journal Article
- Keywords:
mesenchymal stem cells(MSCs);
strain;
c-fos;
c-jun;
c-myc;
verapamil
- From:
Orthopedic Journal of China
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To study the effects of verapamil on the immediate-early gene(IEGs)expression of bone marrow mesenchymal stimulated by mechanical strain,and deduce the effects of calcium ion channel on the early responses of children bone marrow mesenchymal stem cells(MSCs)to mechanical strain in vitro.[Method]MSCs were isolated and cultured.The 3-6th generational MSCs were stimulated in different time by mechanical and(or)verapamil(20?mol/l).Flow cytometry was applied to measure intracellular free Ca2+ at once.Early-response genes /proteins(c-fos,c-jun and c-myc)were examined by RT-PCR and immunocytochemical stain.[Result]The application of mechanical strain increased intracellular c-fos,c-jun and c-myc mRNA /protein levels.The resulting effect is partially inhibited before MSCs were preincubated with verapamil(P
