Effect of XLGB treatment in the rabbit anterior cruciate ligament transaction models of osteoarthritis
- VernacularTitle:仙灵骨葆对兔膝骨性关节炎治疗作用的研究
- Author:
Tan CHENG
;
Liu ZHANG
;
Zhen LI
- Publication Type:Journal Article
- Keywords:
xianlinggubao;
osteoarthritis;
chondrocyte;
subchondral bone;
BMP-2;
MMP-13
- From:
Orthopedic Journal of China
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To assess the protective effects of Xianlinggubao on articular cartilage and subchondral bone in experimental osteoarthritis induced by anterior cruciate ligament transaction (ACLT)in rabbits. [Methods]Thirty healthy Japanese white rabbits were divided randomly into three groups:sham group,ACLT+XLGB group and ACLT+ NS group. Rabbits in ACLT+XLGB group and ACLT+NS group received anterior cruciate ligament transection(ACLT)on the right knee. Rabbits in sham group received untreatment as control. ACLT+XLGB group received a daily administration of XLGB at a dose of 125 mg/kg of body weight for 6 weeks.In contrast,ACLT+NS group received saline at the same dose. All rabbits were sacrificed at 6 weeks after operation. The right femora were removed,measured for bone mineral density (BMD),and then were observed for the macro-pathologic changes with HE staining and graded by Mankin's scale. Expression levels of BMP-2 and MMP-13 were detected by immunostaining. The subchondral bone of right tibial plateau was cut and stained for the measurement of bone histomorphometry.[Results](1)The Mankin's score in ACLT+ XLGB group was significantly lower than that in ACLT+ NS group (P
