Effect of constant magnetic field on metal ion Co~(2+),Cr~(3+) induced TNF-? secreted by human mononuclear cells
- VernacularTitle:恒磁场对金属离子钴铬作用下人单核细胞分泌肿瘤坏死因子的影响
- Author:
Min DAI
;
Jianwei XIONG
;
Ping ZHAN
- Publication Type:Journal Article
- Keywords:
constant magnetic field;
tumor necrosis factor;
metal ion;
aseptic loosening
- From:
Orthopedic Journal of China
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To detect the effect of constant magnetic field on metal ion Co2+,Cr3+ induced TNF-? secreted by human mononuclear cells,and to search a method for prevention and treatment of aseptic loosening. [Methods]CoCl2 powder and CrCl3 powder were dissolved in the asepsis injecting water. Mononuclear cells from human peripheral blood,were taken and cultured with Co2+,Cr3+ ions in different magnetic field of 10Gs,100 Gs,1000Gs for 12,24 and 48 hs. There were nine groups:control group,Co2+ group,Cr3+ group,Co2+andCr3+ with various intensities of constant magnetic field,respectively.ELISA method was applied to detect tumor necrosis factor (TNF-?) in serum via the absorbance (A).[Results]Co2+ and Cr3+ ions stimulated human mononuclear cell to secrete TNF-? (P
