Antisense oligonucleotide targeting survivin gene induces cell apoptosis in salivary adenoid cystic carcinoma
- VernacularTitle:survivin反义寡核苷酸对人涎腺腺样囊性癌细胞凋亡的影响
- Author:
Xu WANG
;
Wei CUI
;
Fusheng DONG
;
Hong SHI
- Publication Type:Journal Article
- Keywords:
Surviving;
Antisense oligonucleotide;
Salivary adenoid cystic carcinoma;
Apoptosis
- From:
Journal of Practical Stomatology
1996;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of survivin antisense oligonucleotide (ASODN) on expression of survivin and ACC-M cell apoptosis. Methods: A phosphorothioate antisense oligonucleotide (ASODN) of specific target survivin was designed and synthesized and then transferred to ACC-M cell line by lipofectin. At the same time blank control group, sense oligonucleotide (SODN) group were set up for comparison. MTT assay was used to detect cytotoxicity. Apoptosis was observed by flow cytometry. Survivin expression was determined by RT-PCR and Western-Blotting. Results: Compared with control group and SOND group, in ASODN groups, the expression of survivin mRNA and protein were obviously weak, apoptosis rate apparently increased, cells growth was inhibited. There was no obviously difference in SODN and control groups.Conclusion:ASODN can down-regulate the expression of survivin gene in ACC-M cell line specifically. It plays an important role in inducing tumor apoptosis and suppressing cell proliferation.
