Effects of Different Reperfusion Sequence on Hepatic Ischemia-Reperfusion Injury
- VernacularTitle:肝门阻断时肝动脉与门静脉不同开放时序对肝脏缺血再灌注的影响
- Author:
Guowen GU
;
Xiangcheng LI
;
Yong SUN
- Publication Type:Journal Article
- Keywords:
Reperfusion injury;
Hepatic artery;
Portal vein;
Oxygen radical
- From:
Chinese Journal of Bases and Clinics in General Surgery
2003;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of different reperfusion sequence on hepatic warm ischemia-reperfusion injury and its related mechanisms.Methods Ninety-six healthy male Sprague Dawley rats were randomly divided into 6 groups by using random digits method(n=16,each): Sham operation group,only shammed operation for negative control;the other 5 groups were all experimental groups,which were divided according to different reperfusion sequences of portal vein and hepatic artery: reperfusion first through the portal vein for 1 min with subsequent full reperfusion group,reperfusion first through the portal vein for 2 min with subsequent full reperfusion group,reperfusion first through the hepatic artery for 1 min with subsequent full reperfusion group,reperfusion first through the hepatic artery for 2 min with subsequent full reperfusion group,simultaneous reperfusion through the portal vein and hepatic artery group.Each group was further randomly divided into two subgroups(n=8,each) for sample collection at 2,4 hours after reperfusion respectively.Serum alanine aminotransferase(ALT),aspartate aminotransferase(AST) and malondialdehyde(MDA),superoxide dismutase(SOD) and glutathion(GSH) in hepatic tissue were detected respectively.HE staining of histopathologic slides was used to observe the morphological changes of hepatic tissue.TUNEL method was used to assess the apoptosis index(AI) of hepatocytes.Results The liver of rat was approximately normal in the sham operation group with lower levels of ALT,AST,MDA and AI,and higher levels of SOD and GSH as compared with all the experimental groups(P
