The expression of ASC in the non-apoptotic,non-necrotic cell death of neutrophils induced by ONO-AE-248
- VernacularTitle:ASC在ONO-AE-248诱导的中性粒细胞非凋亡、非坏死性死亡中的表达
- Author:
Yanling HU
;
Guoli LI
;
Jiajia LIU
- Publication Type:Journal Article
- Keywords:
Apoptosis-associated speck-like protein(ASC);
ONO-AE-248;
Neutrophils;
Non-apoptotic,Non-necrotic neutrophil death;
Apoptosis
- From:
Chinese Journal of Immunology
1985;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of apoptosis-associated speck-like protein(ASC) in non-apoptotic,non-necrotic cell death of neutrophils induced by ONO-AE-248.Methods:The morphological changes of neutrophil nuclei and DNA fragmentation of the cells were examined by confocal microscopy and terminal-deoxynucleotidyl transferase mediated nick end labeling(TUNEL).Neutrophils were stimulated with medium alone,LPS,TNF-? or ONO-AE248 respectively.Then expression of ASC was detected by Western blot.Results:ONO-AE-248-induced neutrophil death was negative in TUNEL assay.Western blot showed that the expression of ASC protein was down-regulated by ONO-AE-248.Conclusion:The DNA degradation of the neutrophils when stimulated by ONO-AE-248 is probably different from that of the typical apoptosis.ASC might be a crosslink point between the ONO-AE-248-induced neutrophil death and spontaneous apoptosis of neutrophils.
