Modulation of Caspase expression by up-regulating histone acetylizad level with VPA
- VernacularTitle:应用VPA调节组蛋白乙酰化修饰对肿瘤细胞Caspase的影响
- Author:
Xia ZHAO
;
Changwen SHI
;
Jingjie SUN
;
Lili CAO
;
Jie LI
;
Zhenhai YU
- Publication Type:Journal Article
- Keywords:
Histone acetylase;
Histone deacetylase inhibitor;
Valproic acid(VPA);
Heptocellular carcinoma;
Gastric carcinoma;
Breast cancer;
Apoptosis;
Caspase regulation
- From:
Chinese Journal of Immunology
1985;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate modulation of a specific HDAC inhibitor,Valproate acid sodium(VPA),on expression of Caspase3,Caspase8,Caspase9 by inhiting HDAC,as well as apoptosis rate of cancer cells treated with VPA and the specific inhibitors of Caspase3,Caspase8,Caspase9.Methods:Heptocellular carcinoma cells-HepG2,gastric carcinoma cells-BGC-823 and breast cancer cells-MCF-7 were cultured with 0.75-4.0 mmol/L of VPA for 48 hrs in vivo,apoptosis was analyzed by flow cytometry with Annexin V/PI assay.The activities and protein expressions of Caspase3,Caspase8,Caspase9 were detected by spectrophotometry and indirect immunofluorescence technique.Results:Contrary to control groups,VPA at concentrations between 0.75 and 4.0 mmol/L induced a significant apoptosis in HepG2,BGC-823,MCF-7 cells(P0.05).The apoptosis rates of cancer cells treated with VPA and specific inhibitors of Caspase3,Caspase9 together was lower than in the groups with VPA treatment singlely(P
