Simultaneous Determination of Homovanillia Acid and Vanillymandelic Acid in Human Urine by HPLC
- VernacularTitle:尿中高香草酸和香草扁桃酸的高效液相色谱测定法
- Author:
Wenjing ZHANG
;
Jie LI
;
Zhihu ZHANG
- Publication Type:Journal Article
- Keywords:
Chromatography,liquid;
Homovanillic acid;
Vanilmandelic acid
- From:
Journal of Environment and Health
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC-FLD method for simultaneous determination of homovanillic acid(HVA) and vanilmandelic acid(VMA) in human urine.Methods After being filtered with 0.45 ?m membrane,the samples of urine were injected directly into an ODS column(250 mm?4.6 mm,5.0 ?m) at the room temperature.The samples of urine were carried with the mobile phase comprised of methanol-0.1mol/L phosphate buffered solution(20:80,V/V).The flow rate was 1 ml/min,the injection volume was 10 ?l,the detection was taken at ?ex=277 nm,?em=320 nm.Results The determination was finished in 15 min,the retention time was 3.18 min for VMA and 6.72 min for HVA respectively.The detection limit of HVA was 0.15 ?g/ml,the linear range was 0-25 ?g/ml,the recovery rates were between 84.53%-106.1%,the relative standard deviation(RSD)
