Effects of cell hypoxia on connexins in unstable bladder smooth muscle cells
- VernacularTitle:缺氧对膀胱平滑肌细胞缝隙连接蛋白表达的影响
- Author:
Zhenbao ZHANG
;
Hao LIN
- Publication Type:Journal Article
- Keywords:
smooth muscle cell;
cell hypoxia;
unstable bladder;
connexin;
bladder outlet obstruction
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the pathogenesis of unstable bladder with partial bladder outlet obstruction through the experiment based research on connexins in both unstable bladder group and cell hypoxia group.Methods Unstable bladder rat models were established and smooth muscle cells were cultured in vitro.Then cell anoxia was induced.RT-PCR and Western blot were used to quantify the connexins.Results The mRNA levels of Cx40,Cx43 and the Cx43 protein expressed in the smooth muscle cells from the unstable bladder group and cell hypoxia groups were all higher than those in the control groups(the sham-operation group and normoxia groups)(P
