Effect of anti-transplanted tumor in nude mice by DC induced in vitro
- VernacularTitle:树突状细胞对裸鼠结肠癌移植瘤的抗癌效应
- Author:
Zibiao HE
;
Weiming YANG
;
Baoting JIAO
;
Hui SONG
- Publication Type:Journal Article
- Keywords:
dendritic cell;
colon carcinoma;
immunotherapy;
nude mouse;
anti-tumor
- From:Journal of Xi'an Jiaotong University(Medical Sciences)
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the immune response of dendritic cells(DC)modified with tumor antigen and SEB to transplanted tumor in nude mice.Methods Nude mice models were established with human colon cancer cells inoculated subcutaneously,grouped into control group,therapy group and retherapy group;DC was isolated and purified from PBMC of colon carcinoma patient to activate T lymphocyte with DC modified by tumour antigen and SEB.The T lymphocyte was implanted to the models to inhibit the growth of implanted tumor through tail vein.Tumor formation,growth and size,nude mice feeding and activity status were observed.Results The subcutaneously xenotransplanted tumor model of human colon carcinoma in nude mice was successfully established.After colon cancer cells had been inoculated in nude mice for 60 days,volume of transplanted tumor in the control group was(3 899?22)mm3,therapy group(1 301?16)mm3,and retherapy group(392?18)mm3.Compared with the control group,the tumor in therapy group and retherapy group could grow slowly(P=0.000).Compared with that in the therapy group,the tumor growth in retherapy group became obviously slower(P=0.000).Conclusion The immune response induced by DC can inhibit the growth of transplanted tumor in nudes mice,which may provide a new therapy for tumor.
