Expression of DNMT1,?-catenin and P-GSK-3? in colon carcinoma
- VernacularTitle:DNMT1、?-catenin和P-GSK-3?在结肠癌组织中的表达
- Author:
Yongchang WEI
;
Dalin HE
;
Jiahui ZHAO
;
Jirong BAI
- Publication Type:Journal Article
- Keywords:
DNA methyl transferase 1(DNMT1);
phospho-glycogen synthase kinase-3 beta(P-GSK-3?);
?-catenin;
colon carcinoma
- From:Journal of Xi'an Jiaotong University(Medical Sciences)
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine DNMT1,?-catenin and P-GSK-3? expressions in tissues of colon carcinoma compared with tumor clinicopathological parameters including differentiation and metastasis.Methods SYBR Green real-time PCR and immunoblotting method were used to detect these gene expressions in 62 tissues of colon carcinoma and 21 tissues of normal colon.Results Overexpressions of DNMT1,?-catenin,and P-GSK-3? were found in colon carcinoma tissues.The expression of DNMT1 was found to be higher in poorly differentiated tissues.The results also demonstrated statistical significance in the expression of ?-catenin involving differentiation and metastasis,but no statistical significance in the expression of P-GSK-3?.Conclusion These results show that DNMT1,?-catenin and P-GSK-3? expressions are regulated throughout colon cancer progression.
