Enhancement of the cytotoxic effect of cytokine induced killers by dendritic cells pulsed with astragalus polysaccharides
- VernacularTitle:黄芪多糖诱导的树突状细胞增强CIK细胞的杀伤作用
- Author:
Song ZHANG
;
Xiaoyan MU
;
Hongmei WANG
;
Shujuan JIANG
- Publication Type:Journal Article
- Keywords:
Dendritic cell(DC);
Cytokine induced killer;
Astragalus polysaccharides
- From:
Chinese Journal of Immunology
1985;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effects of Astragalus polysaccharides on antigen presentation of dendritic cells(DC).Methods:DC and CIK cells were generated by culturing PBMC of healthy blood donor.The typical DC and DC pulsed by Astragalus polysaccharides were co-cultured with CIK cells,respectively.Then the changes in the phenotypes of the DCs pulsed with Astragalus polysaccharides were determined.Cell surface markers were analysed by FACS method.IFN-? and IL-12 secreted by co-cultured cells were detected by ELISA.The cytotoxicity of effector cells on A549 cells in vitro were measured by MTT assays.Results:Astragalus polysaccharides could increase the expression of CD40,CD80 and HLA-DR on DC surface.The Astragalus polysaccharides pulsed DC-CIK cells resulted in an enhanced killing activity to A549 cells than that of unpulsd DC-CIK cells(P
