Construction of soluble anti-digoxin ScFv antibodies and diabody
- VernacularTitle:抗地高辛人源小分子抗体的构建
- Author:
Yuanyuan QIAO
;
Yan WANG
;
Xiaohang ZHAO
;
Guomin ZHANG
;
Yuping CHEN
- Publication Type:Journal Article
- Keywords:
Anti-digoxin;
ScFv;
Diabody
- From:
Chinese Journal of Immunology
1985;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To clone soluble human anti-digoxin antibodies from large phage antibody library and construct a vector that expresses diabody.Methods:Soluble ScFvs were prepared through infecting E coli.HB2151 with the selected phage antibodies and induced with IPTG.The diabody vector was modified by enzyme digestion and checked by SDS-electrophoretogram.Results:It was showed by ELISA that soluble ScFv had specific binding ability to digoxin.The vector to express a soluble diabody was obtained by genetic modification,which was shown by Western blot.Conclusion:Soluble human anti-digoxin antibodies were successfully obtained from phage antibodies.The vector is efficient in creating diabody.
