The mechanism for dexamethasone-induced apoptosis of NK-92MI cells
- VernacularTitle:地塞米松诱导NK-92MI细胞株凋亡机制的研究
- Author:
Jiayi HOU
;
Weixin FU
;
Xiukun JIA
;
Bo QIN
;
Zaifu LIANG
- Publication Type:Journal Article
- Keywords:
Dexamethasone;
NK-92MI cell;
Apoptosis;
Bcl-2;
Bax
- From:
Chinese Journal of Immunology
1985;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of dexamethasone(DEX)on the cytotoxicity and apoptosis of NK-92MI cells and the mechanisms involved.Methods:NK-92MI cells were treated with different doses of DEX.The proliferative rate and cytotoxicity of the NK-92MI cells were detected by MTT colorimetry.The cell apoptotic rate was observed by flow cytometry with Annexin V and propidium iodide(PI)double staining.The expression of apoptosis-related gene,Bcl-2 and Bax was detected by RT-PCR.Results:After treated with 1?10-8mol/L to 1?10-3mol/L of DEX for 24 h,48 h and 72 h,the proliferation of NK-92MI cells was significant inhibited(P
