Experimental study of expression of BMP-2 and VEGFmRNA in local femoral head of nontraumatic osteonecrosis of femoral head
- VernacularTitle:VEGFmRNA与BMP-2在非创伤性股骨头坏死中表达的研究
- Author:
Zhigang BAI
;
Wanlin LIU
;
Xiulan SU
- Publication Type:Journal Article
- Keywords:
nontraumatic osteonecrosis of femoral head;
bone morphogenic protein-2;
vascular endothelial growth factor;
human body sample
- From:
Orthopedic Journal of China
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To explore the change and its significance of the expression of vascular endothelial growth factor(VEGF) messenger RNA and bone morphogenic protein-2(BMP-2) of the local femoral head in the nontraumatic osteonecrosis of femoral head(NONFH).[Method]Sixteen samples of femoral heads of NONFH were collected as the experimental group and fresh 10 samples of femoral heads of femoral neck fracture as control group,overall examples were collected from total articular replacement arthroplasty.The samples were splitted in coronal plane and get one bone block from necrosis area and another one from healthy area,make them into microtome section after the process of immobility and decalcification.Their pathological change was observed by using optical microscope and electron microscope and detect the expression of BMP-2 and VEGFmRNA in femoral head through making use of immunohistochemistry and in-situ hybridization technique.[Result]The organization structure of experimental group was disorganized,cracked and the bone trabecula was rarefactive and non-intact and there was a great number of empty lacuna in bone trabecula.While there was the reverse situation in the control group.The intensity and area of positive expression of BMP-2 and VEGFmRNA in femoral head of the experimental group were lower than that of control group obviously.The result showed statistical significance(P
