Evaluation of the cytotoxicity of four dental alloys on human gingival fibroblasts
- VernacularTitle:4种铸造合金对人牙龈成纤维细胞的毒性研究
- Author:
Yang YANG
;
Juanjuan JI
;
Bei PENG
;
Xiaoyu ZHANG
;
Xiangyun WANG
- Publication Type:Journal Article
- Keywords:
Dental Alloys;
Cytotoxicity;
MTT assay;
Flow Cytometry
- From:
Journal of Practical Stomatology
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To identify the cytotoxicity of four dental casting alloys on human gingival fibroblasts.Methods: MIT assay method was used to evaluate the cytotoxicity of four alloys on human periodontal ligament fibroblasts(HPDLFs) isolated from healthy periodontal ligaments of subjects on the 2nd d,4th d and 7th d of culture.Flow Cytometry(FCM) was used to evaluate the cytotoxicity of four alloys to human gingival fibroblasts.Results: It was found that there was no apparent cytotoxicity of all the four alloys.Conclusion:MTT and FCM methods show the same results.These four alloys have good biocompatibilities.
