Preparation and purification of polyclonal antibodies against human Fc?/?R and the polymeric immunoglobulin receptor
- VernacularTitle:人Fc?/?R和Poly Immunoglobulin Receptor多克隆抗体的制备及鉴定
- Author:
Lijun YANG
;
Wei ZHANG
- Publication Type:Journal Article
- Keywords:
Fc?/?R;
pIgR;
Polyclone antibodies
- From:
Chinese Journal of Immunology
1985;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To generate rabbit anti-human Fc?/?R and Poly Immunoglobulin Receptor(pIgR) polyclonal antibodies.Methods:The EC12 domain of human Fc?/?R and the D1 domain of pIgR were expressed as including bodies in Escherichia coli.The purified proteins were used to immunize rabbits to generate polyclonal antibodies.The immune sera were characterized by ELISA and Western blot analysis,then absorbed with cross-action and purified by antigen linked beads.Results:The titers of antiserum were 1∶6 4000 and 1∶25 6000,separately.There was little cross-reaction between the two immune sera.Conclusion:The polyclonal antibodies against human Fc?/?R and pIgR without cross-reaction were successfully generated and could be useful tools to study the relationship between the two receptors.
