Antisense oligonucleotides targeting TNF-? suppress on Co-Cr-Mo particle-induced osteolysis
- VernacularTitle:靶向TNF-?的反义寡核苷酸治疗人工关节磨损微粒诱导的骨溶解的实验研究
- Author:
Rui WANG
;
Ting GUO
;
Xiaofeng ZENG
- Publication Type:Journal Article
- Keywords:
antisense oligonucleotides;
osteoclast;
loosening;
replacement;
tumor necrosis factor
- From:
Orthopedic Journal of China
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To investigate the effect of a single subcutaneous dose of an antisense oligonucleotide(ASO) on particle-induced osteolysis.[Method]The murine calvaria osteolysis model was utilized in ICR mice.Bone resorption was measured with the toluidine blue staining.Osteoclasts were detected by tartrate resistant acid phosphatase(TRAP) staining assay and were quantified by a TRAP quantification kit.[Result]Bone resorption was 0.347 ? 0.09 mm2 in animals with particle implantation,and decreased to 0.123 ? 0.05 mm2 and 0.052 ? 0.02 mm2 after ASO treatment in low and high doses,respectively.The bone resorption was reestablished in animals given an additional TNF-?.The number of osteoclasts in animal calvaria treated with ASO was reduced obviously compared with those untreated animals and the quantification results indicated that about 90% osteoclastgenesis was suprressed by the ASO.Additionally,the osteoclastgenesis was reestablished by the addition of TNF-?.[Conclusion]An antisense oligonucleotide targeting an inflammatory factor,TNF-?,has been to suppress the osteolysis induced by particle for the first time.This new finding holds a great promise.It is a therapeutic strategy for the component loosening.
