Experimental study on inducement of rat small bowel trans-plantation tolerance by immature dendritic cell cultivated and expansed in vitro
- VernacularTitle:未成熟DC体外扩增诱导大鼠小肠移植免疫耐受的实验研究
- Author:
Zhaoyang LI
;
Changle RUAN
;
Shuguang ZHANG
;
Guangxin LI
- Publication Type:Journal Article
- Keywords:
Transplantation?Small bowel?Dendritic cell?Immune tolerance?Rat
- From:
Chinese Journal of Current Advances in General Surgery
1998;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To discuss the feasibility of immune tolerance in rat small bowel trans-plantation induced by immature dendritic cells isolated from SD and Wistar rat. Methods: Dendritic cells isolated from liver or bone marrow of inbred strain Wistar and SD rat were cultured for five to nine days. A week before operation, the recipient rats were injected 2?106 inmature DCs from vena dorsalis penis and observed. The models of heterotopic small bowel transplantation (SBT: SD→ Wistar)were established. Acute immuno- rejection levels were evaluated by HE. Results: Varying degrees of rejection were observed in 3, 5, 7 days after operation. Conclusion: Immature DC can induce allogenic immunological tolerance of rat small bowel transplantation.
