Expression of Cad-Ⅱ gene in bone marrow mesenchymal stem cells transfected with Cad-ⅡcDNA after autografted into the bone defects
- VernacularTitle:Cad-Ⅱ基因转染的兔骨髓间充质干细胞自体移植术后的表达研究
- Author:
Qiang XIANG
;
Congying DENG
;
Wenjie ZHENG
- Publication Type:Journal Article
- Keywords:
Cad-Ⅱ;
bone marrow mesenchymal stem cell;
bone defect
- From:
Orthopedic Journal of China
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To study the expression of Cad-Ⅱ gene in bone marrow mesenchymal stem cells(MSCs) transfected with Cad-ⅡcDNA after autografted into the bone defects. [Method]The experimental model of ilium segment defect was established in 20 Japanese white rabbits.The rabbit MSCs were isolated,cultured and expanded in vitro,and then the MSCs,transfected with Cad-Ⅱ and compounded with collagen sponge were autografted into the ilium segment defect.At 4 weeks of operation,the MSCs/ collagen sponge were excised,and the expression of Cad-Ⅱ was evaluated with RT-PCR and immunohistochemical methods.[Result]All of the bone defects treated with implants exhibited new bone formation at 4 weeks postoperatively.In the transfection group,Cad-Ⅱ gene mRNA expression was higher than that in the control group(P
