Effect of mesenchymal stem cells from rat bone marrow on immunological tolerance
- VernacularTitle:大鼠骨髓间充质干细胞对免疫耐受的作用
- Author:
Jinsong WANG
;
Xiaoye QIN
;
Zhenbo SHU
;
Yan SONG
- Publication Type:Journal Article
- Keywords:
Bone mesenchymal stem cells;
Cell isolation;
Cell culture;
Cell identification;
CD4+CD25+Treg
- From:
Chinese Journal of Immunology
1999;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investiate biological properties of Mesenchymal stem cell(BMSC) from rat bone marrow in vitro,and their effects in inducing immune tolerance.Methods:BMSCs were obtained from rat bone marrow by density gradient centrifugation and selected by cell attachment.The morphology of BMSCs was observed by electron microscope.The expression of surface molecules of CD34 and CD44 was analyzed by flow cytometry.The cells were injected ex vivo intravenously after in vitro culture and then CD4+ CD25+ Treg ratio were determined.The proliferation of thymic cells was estimated by 3H-TdR incorporation method.Results:BMSCs were fibroblast-like cells.The expression ratio of CD44 in BMSCs was 98%,but expression of CD34 was negative.After administration of BMSCs,the ratio of CD4+CD25+Treg(2.5%?0.69%) from peripheral blood of rats was higher than in normal group(0.8%?0.14%) and PBS control group(1.0%?0.23%),P
