Empirical study of titration of IgG antibody against blood group in pregnant women by using domestic microcolumn gel Coombs'test
- VernacularTitle:国产微柱凝胶免疫检测试剂卡检测孕妇IgG血型抗体效价的实验研究
- Author:
Lingbo LI
;
Dongqian WANG
;
Yunsheng CHEN
;
Ning LI
;
Weijia CHEN
- Publication Type:Journal Article
- Keywords:
Microcolumn gel Coombs test;
test tube Coombs test;
IgG antibody titre
- From:
Chinese Journal of Immunology
1999;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare microcolumn gel Coombs test and test tube Coombs test for IgG anti-A and anti-B titre in serum of pregnant women with blood type O.The critical titre for IgG anti-A and anti-B should be established in domestic microcolumn gel Coombs' test.Methods:524 blood samples of pregnant women with blood type O,whose husbands were of blood type A or B,were detected simultaneously by domestic microcolumn gel Coombs' test and test tube Coombs test.The results were analyzed using paired "t " test,"?2 " test and regression analysis.Results:IgG anti-A mean titres determined by the two methods separately were 249.98 and 120.85,and IgG anti-B mean titre were 156.98 and 76.38.IgG anti-A and anti-B titration in domestic microcolumn gel Coombs'test showed significantly higher titres(mean 2.07 fold and mean 2.06 fold) than in test tube Coombs test in all samples studied(t=19.64,P
