Transcriptional regulation of vascular endothelial growth factor by estradiol and tamoxifen in breast cancer
- VernacularTitle:雌激素及其拮抗剂对乳腺癌血管内皮生长因子的转录调节
- Author:
Xinmei KANG
;
Qingyuan ZHANG
;
Wenhui ZHAO
- Publication Type:Journal Article
- Keywords:
estradiol;
tamoxifen;
VEGF;
breast neoplasm
- From:
China Oncology
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Background and purpose:Angiogenesis plays an important part in growth and metastases of breast cancer. Nowadays researchers put more concern about whether endocrine therapy adjusts tumor angiogenesis in breast cancer. Here we investigated the regulation of vascular endothelial growth factor(VEGF) expression in breast cancer in response to various stimuli:estradiol(E2) ,ICI182780 and tamoxifen. Methods:We studied the regulation of VEGFmRNA expression in MCF-7 cells stimulated by E2 at different doses and duration by semiquantitative RT-PCR. When ICI182780 and Tamoxifen used alone or in combination with E2,the expression of VEGFmRNA was detected respectively. Results:Dose-dependent experiments indicated that the maximum VEGF mRNA level(0.125?0.006) -(0.112?0.014) was obtained at 1-10 nmol/L.The response of VEGFmRNA expression to E2(1 nmol/L) occurred in a time-dependent manner;the level of VEGF transcripts(0.105?0.009) signifi cantly increased within 2 h and reached a maximum at 6 h(0.140?0.024)(P
