Effect of low intensity pulsed ultrasound on repairing bone defect by cell-scaffold complex composed by rabbit bone marrow stromal cells and ?-tricalcium phosphate
- VernacularTitle:低强度脉冲超声波影响兔骨髓基质干细胞与?-磷酸三钙复合修复骨缺损的实验研究
- Author:
Wei LIANG
;
Sanhuai GOU
;
Yueping OUYANG
- Publication Type:Journal Article
- Keywords:
bone tissue engineering;
cell-scaffold complex;
bone defect;
low intensity pulsed ultrasound
- From:
Orthopedic Journal of China
2006;0(23):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To explore the effect of low intensity pulsed ultrasound(LIPUS)on the ability of repairing rabbit radius defect by cell-scaffold complex composed with bone marrow stromal cells(BMCs)and ?-tricalcium phosphate(?-TCP).[Method]Bone marrow of a rabbit was drawn and cultured by differentiation culture medium to harvest BMCs and the third generation BMCs were cultivated on ?-TCP for one week in vitro.Bilateral distal radial defects were made using a 1-mm saw in 20 mature New Zealand white rabbits.The cell-scaffold complexs stimulated by LIPUS were implanted in rabbit radial defects(cell-scaffold complexs without stimulation as control).All the rabbits were killed at 4 or 8 weeks after operation.The defect healing state was observed with X-ray and histological technic.The gray density of CA was analyzed by computer image analysis system.[Result]It was found that the callus in the experimental group grew more quickly and apparently than that in the control group.At 4 weeks after operation,there was significantly statistical difference(P0.05).Histological slices in the experimental group showed that the absorption,organization of hematoma,the formation of trabeculae and matrix preceded those in the control group.There were more fibroblast,osteoblasts and callus in the experimental group.[Conclusion]Cell-scaffold complex composed with BMCs and ?-TCP stimulated by LIPUS can repair bone defect more effectively than that without LIPUS stimulation at the early stage of healing,but the effect tend to decrease at the late stage.
