Values of radionuclide bone imaging to dynamically monitor revascularization of tissue-engineered bone graft in repairing bone defect of rabbits
- VernacularTitle:放射性核素骨显像动态检测组织工程化骨血管化的价值
- Author:
Zhihou FU
;
Aimin WANG
- Publication Type:Journal Article
- Keywords:
mesenchymal stem cells;
revascularization;
mesenchymal stem cell;
bone defect;
tissue-engineered bone
- From:
Orthopedic Journal of China
2006;0(22):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To dynamically monitor revascularization and metabolism of tissue-engineered bone graft in repairing bone defects by radionuclide bone imaging.[Method]Twenty-four healthy New Zealand rabbits were studied.Mesenchymal stem cells(MSCs) were separated and cultured from bone marrow.Cancellous bone defects(0.6 cm?1.2 cm) were created on bilateral femoral condyles in rabbits.The compound of coral hydroxyapatite ceramic(CHA) and defects were treated with the induced osteoblasts inplanted into the left defects(experimental group).While CHA alone was used for the right(control group).The counts of regions of interest(ROI) was evaluated by radionuclide bone imaging at 4,8 and 12 weeks postoperatively.[Result]ROI in the experimental group was significantly higher than that of control group at 4,8 and 12 weeks postoperatively.ROI in either group had a tendency to increase with time.Eight weeks postoperatively,ROI in experimental group tended to increase at a lower speed,while ROI in control group had a tendency to increase at a higher speed.ROI in either group reached a peak at 12 weeks postoperatively.[Conclusion]The tissue-engineered bone graft is an effective method for repairing cancellous bone defects.Radionuclide bone imaging can dynamically monitor the changes of revascularization and metabolism of tissue-engineered bone graft.
