Influence of TNF-? on the expression of secretory component in Caco-2
- VernacularTitle:肿瘤坏死因子对肠上皮细胞分泌片的影响
- Author:
Dongyan LIU
;
Pei LIU
- Publication Type:Journal Article
- Keywords:
Secretory component(SC);
Polymeric Ig receptor (pIgR);
TNF-?;
Caco-2
- From:
Chinese Journal of Immunology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the influence of TNF-? on the expression of secretory component (SC) in Caco-2 cells.Methods:Immunocytochemistry,ELISA,Western blot and quantitative real-time PCR were used to test SC-positive cells of Caco-2,free SC in culture supernatants,protein and mRNA expression of SC in the cells.Results:The increase of SC-positive cells,free SC in culture supernatants,SC protein expression and expression of SC genes in Caco-2 cells was found under stimulation of TNF-? treatment compared with control(P
